Defining HLA-II ligand processing and binding rules with mass spectrometry enhances cancer epitope prediction
نویسندگان
چکیده
(Immunity 51, 766–779.e1–e17; October 15, 2019) The proteomics data deposition for this study (located at ftp://massive.ucsd.edu/MSV000083991) has been corrected to include all MAPTAC-profiled alleles. complete dataset (including previously uploaded files) is now located under "updates/2020-10-05_JAbelin_e5cb28f1/.” An associated "README" file (at other/README_RawAndSearchFileLocation.txt) details the locations of file-to-sample mapping file, raw spectra files, and peptide search result files. In addition, software also source code (now available https://bitbucket.org/dharjanto-neon/neonmhc2) that was used train online predictor (neonmhc2.org). authors apologize inconvenience caused. Defining HLA-II Ligand Processing Binding Rules with Mass Spectrometry Enhances Cancer Epitope PredictionAbelin et al.ImmunitySeptember 5, 2019In BriefDespite their role in directing T cell responses, epitopes remain difficult predict, hindering therapeutic potential. Abelin al. develop proteomic strategies resolve diverse motifs pinpoint tumor are presented by professional APCs. These enable improved epitope prediction targeting. Full-Text PDF Open Archive
منابع مشابه
T-Epitope Designer: A HLA-peptide binding prediction server
UNLABELLED The current challenge in synthetic vaccine design is the development of a methodology to identify and test short antigen peptides as potential T-cell epitopes. Recently, we described a HLA-peptide binding model (using structural properties) capable of predicting peptides binding to any HLA allele. Consequently, we have developed a web server named T-EPITOPE DESIGNER to facilitate HLA...
متن کاملDefining the role of mass spectrometry in cancer diagnostics.
The prospect of uncovering the information contained in the human genome garnered such great promise that it attracted a synergistic blend of researchers from multiple disciplines. Notably, this group included molecular biologists, clinician-scientists, computational mathematicians, and industry input via engineers and, in several cases, management expertise in the form of leadership. This syne...
متن کاملA MAGE-A1 HLA-A*0201 Epitope Identified by Mass Spectrometry
Peptides presented by HLA-A*0201 molecules on the surface of the human breast carcinoma cell line KS24.22 after IFN-g induction were analyzed by the “Predict-Calibrate-Detect” approach, which combines epitope prediction and high-performance liquid chromatography mass spectrometry. One of the predicted epitopes, MAGE-A1278–286 (KVLEYVIKV), was found to be presented by HLA-A*0201, with an estimat...
متن کاملQuantifying epitope presentation using mass spectrometry.
Understanding the absolute quantities of MHC-bound epitopes (pMHC) presented on the surface of cells has long been a critical missing element in our knowledge of antigen presentation to T cells. Until recently, attaining such information has been restricted to the use of pMHC complex-specific monoclonal antibodies or T cell assays probing fractionated peptides eluted from cells. Although succes...
متن کاملProtein epitope mapping by mass spectrometry.
A mass spectrometric method is described for the rapid mapping of linear epitopes in proteins that are bound by monoclonal antibodies. The method consists of three steps. In the first step, an antigen protein is digested by a proteolytic enzyme to produce an appropriate set of peptide fragments. In the second step, peptide fragments containing the linear epitope are selected and separated from ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Immunity
سال: 2021
ISSN: ['1097-4180', '1074-7613']
DOI: https://doi.org/10.1016/j.immuni.2020.12.005